Even though molecular biology has long been a basic tool in biomedical research, scientists still face the question of why certain molecular biology methods are used for certain experiments. To unlock the mystery, one must first understand the principles behind the methods. Unfortunately, very few molecular biology books have successfully provided satisfactory explanations. This book intends to fill this void by offering topics ranging from basic knowledge to the current state of the art in applied molecular biology. The principles and applications related to each technique included in the text are all described in full detail.

本書特色

　　分子生物學是所有學習生命科學者必修的學分，除了理論架構外，本書最大特色是作者擁有實際臨床實驗經驗，能讓您掌握基因實驗的要訣，作者每年寒暑假皆受邀於國內各大醫學研究中心主講分子生物研討會。

作者簡介

Chao-Hung Lee

　　美國印地安那大學醫學院微生物及免疫研究所博士。

　　美國印地安那大學醫學院病理系教授。

Chapter 1: Nucleic Acid Structure and Properties
Chapter 2: Replication, Transcription and Translation
Chapter 3: Isolation of DNA and RNA
Chapter 4: Enzymes Used in Molecular Biology
Chapter 5: Promoter and Operon
Chapter 6: Electrophoresis
Chapter 7: Cloning
Chapter 8: Nucleic Acid Hybridization
Chapter 9: Library
Chapter 10: Restriction Mapping
Chapter 11: DNA Sequencing
Chapter 12: Cloning Vectors
Chapter 13: Polymerase Chain Reaction
Chapter 14: Expression of Recombinant Proteins
Chapter 15: Mutagenesis, Transgenic Animals and RNA Interference
Chapter 16: Viral Vectors
Chapter 17: Special Techniques
Glossary
Index